For complete protocol details, see Gladstone & Su, 2011.
Step 1: Set up a population cage for breeding.
Step 2: Collect embryos. Left: collection of embryos from grape juice/agar plate. Right: Drosophila embryos.
Step 3: Culture embryos until they become third instar larvae. Left: collection of larvae from culture bottles. Middle: selection of third instar larvae. Right: third instar larvae ready for irradiation and drug treatment.
Step 4: Irradiate and treat with individual compounds. Left: distribution of irradiated larvae into drug vials. Right: three students’ vials each containing irradiated larvae and a single drug mixed into the food.
Step 5: Using fluorescence microscopy, identify GFP in developing pupae to determine genotype.
Step 6: Quantify survival and identify hits.